anti tarc antibodies  (R&D Systems)

Journal: Frontiers in endocrinology

Article Title: CCL17 and CCL22 chemokines are upregulated in human obesity and play a role in vascular dysfunction.

doi: 10.3389/fendo.2023.1154158

Figure Lengend Snippet: FIGURE 1 Circulating CCL17 and CCL22 chemokine levels in morbidly obese patients and age-matched controls. (A) CCL17 and (B) CCL22 levels were measured in plasma samples from obese patients (n = 60) and controls (n = 20). Scatter dot plots showing median with interquartile range. Comparison between groups were made by Mann Whitney test. Spearman test shows a positive correlation between CCL17 and CCL22 with HOMA- IR Index (C, D) and BMI (E, F) (n = 20 control subjects and n = 60 morbidly obese patients).

Article Snippet: Antigen was unmasked with proteinase K (cat#S3020, Dako, Santa Clara, CA) and blocked with 15% horse serum for 1 h. Samples were incubated with the following primary antibodies overnight at 4°C: mouse anti-human CCL17 (1:50, cat#DY364-05, R&D Systems), mouse anti-human CCL22 (1:50, cat#DY336, R&D Systems), goat anti-human CCR4 (1:100, ab1669; Abcam, Cambridge, UK), rabbit anti-human CD3 (1:100, cat#C7930, Sigma-Aldrich, St. Louis, MO), rabbit polyclonal anti-human CD31 (1:50, cat#ab32457, Abcam) and rat anti-human Mac-3 (1:100, cat#sc19991, Santa Cruz Biotechnology, Dallas, TX).

Techniques: Clinical Proteomics, Comparison, MANN-WHITNEY, Control

Journal: Frontiers in endocrinology

Article Title: CCL17 and CCL22 chemokines are upregulated in human obesity and play a role in vascular dysfunction.

doi: 10.3389/fendo.2023.1154158

Figure Lengend Snippet: FIGURE 2 Expression of CCL17 and CCL22 is increased in VCAT from morbidly obese patients. Relative quantification of mRNA levels for (A) CCL17 and (B) CCL22. Comparisons between groups were made by Wilcoxon matched-pair signed-rank test. Values are expressed as mean ± SEM (n = 33). (C) CCL17 and (D) CCL22 chemokine release into conditioned media was determined after 48 h of SCAT and VCAT explant culture. Chemokine secretion is expressed as pg/ml in the supernatant. Values are expressed as mean ± SEM (n = 22). Comparison between groups were made by Mann Whitney test. (E) Immunofluorescence representative images showing colocalization of CCL17 with CD3 (lymphocytes), CD31 (endothelial cells) and Mac-3 (macrophages); or CCL22 with CD3, CD31, Mac-3 in VCAT. Immunoreactivity was visualized using Alexa Fluor 594 (CCL17 and CCL22, red) and Alexa Fluor 488 (CD31, CD3, Mac-3, green) secondary antibodies. Nuclei were stained with Hoechst (blue). Scale bar, 20 mm. Nuclei were stained with Hoechst (blue).

Article Snippet: Antigen was unmasked with proteinase K (cat#S3020, Dako, Santa Clara, CA) and blocked with 15% horse serum for 1 h. Samples were incubated with the following primary antibodies overnight at 4°C: mouse anti-human CCL17 (1:50, cat#DY364-05, R&D Systems), mouse anti-human CCL22 (1:50, cat#DY336, R&D Systems), goat anti-human CCR4 (1:100, ab1669; Abcam, Cambridge, UK), rabbit anti-human CD3 (1:100, cat#C7930, Sigma-Aldrich, St. Louis, MO), rabbit polyclonal anti-human CD31 (1:50, cat#ab32457, Abcam) and rat anti-human Mac-3 (1:100, cat#sc19991, Santa Cruz Biotechnology, Dallas, TX).

Techniques: Expressing, Comparison, MANN-WHITNEY, Staining

Journal: Histopathology

Article Title: The value of thymus and activation related chemokine immunohistochemistry in classic Hodgkin lymphoma diagnostics.

doi: 10.1111/his.14836

Figure Lengend Snippet: Figure 1. A–C, cHL with multiple HRS cells that are CD30-positive (B). TARC IHC in this case shows strong cytoplasmic staining in all the tumour cells (C). D–F, EBV-positive cHL with HRS cells that show diffuse CD30 positivity (E). TARC IHC shows a weak and incomplete stain- ing pattern (F). G–I, Nodular lymphocyte predominant Hodgkin lymphoma with CD20-positive tumour cells (H). TARC IHC in this case is completely negative in all tumour cells (I). cHL, classic Hodgkin lymphoma; HRS, Hodgkin/Reed–Sternberg; IHC, immunohistochemistry; TARC, thymus and activation-related chemokine.

Article Snippet: T A R C I M M U N O H I S T O C H E M I S T R Y Paraffin tissue sections (3 lm) were incubated with polyclonal goat anti-human TARC antibody (1:800; R&D Systems, Minneapolis, MN, USA) on the automated Benchmark ULTRA platform (Ultra CC1, 52 min; Roche, Ventana Medical Systems, Oro Valley, AZ, USA).

Techniques: Staining, Immunohistochemistry, Activation Assay

Journal: Histopathology

Article Title: The value of thymus and activation related chemokine immunohistochemistry in classic Hodgkin lymphoma diagnostics.

doi: 10.1111/his.14836

Figure Lengend Snippet: Figure 2. A, B, reactive lymphadenopathy with multiple CD30-positive immunoblastic cells. TARC proved to be completely negative (B). C, D, Follicular lymphoma with numerous CD30-positive large B cell blasts (C). TARC IHC shows variable staining in part of the blasts (D). E, F, B cell lymphoma unclassifiable with features intermediate between diffuse large B cell lymphoma and classic Hodgkin lymphoma. TARC IHC is weakly positive in part of the tumour cells; the remainder is negative (F). G, H, primary mediastinal B cell lymphoma. TARC IHC shows a weak/variable staining with clear negativity of part of the tumour cells (H). IHC, immunohistochemistry; TARC, thymus and activation-related chemokine.

Article Snippet: T A R C I M M U N O H I S T O C H E M I S T R Y Paraffin tissue sections (3 lm) were incubated with polyclonal goat anti-human TARC antibody (1:800; R&D Systems, Minneapolis, MN, USA) on the automated Benchmark ULTRA platform (Ultra CC1, 52 min; Roche, Ventana Medical Systems, Oro Valley, AZ, USA).

Techniques: Staining, Immunohistochemistry, Activation Assay